Effect of seed inoculation method with Rhizobium species on the germination of alfalfa seeds (Medicago sativa L.)

Inoculation alfalfa seeds with the right Rhizobium strain (nitrogen-fixing bacteria) can stimulate root nodulation and improve plant growth yield. Different inoculation methods are used to inoculate seeds; however, effect of these on germination is not well documented. In present study, was evaluated after using different methods, i.e. Arabic gum solution glucose as adhesive substances surface seeds, charcoal powder sawdust coating materials. The solutions materials were tested separately in combination. Three concentrations (i.e., 5, 12.5, 25%), alongside untreated control. Germination counts started days 3, 4, 6, 8, 10, 12. On day 12, experiment ended final percentage (FGP) index (GI) calculated. Application sole 5 12.5% beneficial inoculated seeds. application seedbed enhances significantly (p < 0.05) compared coal powder. Moreover, a material combination (12.5%) gave higher germination. results demonstrate that either or did harm